H101-OLYMPUS-IX3-SVR 使用说明书-USB迷|专注于互联网分享

2024年9月3日发(作者：势伟彦)

H101-OLYMPUS-IX3-SVR

User Manual

March 2015

H101-OLYMPUS-IX3-SVR

Compatible with the following XY stages Compatible with the following Okolab Controllers



Olympus: IX3-SVR



H101-BASIC-BL



H101-CRYO-BL

User Manual

March 2015

Index

Components 3

Sample Holders and Lid Adapters ............................................................................................. 4

2.1

Available Sample Holders ....................................................................................................... 4

Insertion of the Sample Feedback Temperature Sensor .................................................................. 6

Insertion of Sample Holder into Chamber ................................................................................... 6

Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks ........................................... 8

Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks ........................................................10

Working with MW Plates - Magnetic locks and Chamber riser ..........................................................11

Connectors description ........................................................................................................12

Working with Perfusion ........................................................................................................13

10.

Connection of the Chamber with adapter ..................................................................................13

11.

Connecting the Chamber with XY stage ....................................................................................14

User Manual

March 2015

1. Components and dimensions

H101-OLYMPUS-IX3-SVR includes the following components:



Chamber main body, it is uniformly heated by means water circulation in water tight channels



Cover glass lid. It is uniformly heated by means water circulation in water tight channels



Chamber riser. It is a removable frame increasing the height of the chamber from 26 to 32 mm, often re-

quired when using multi-well (MW) plates

LidAdapter

Lid

Distancebetweenfocalplane

and upperside of the glass

lidwith the chamberriser

Distancebetweenfocalplane

and upperside of the glass

lidwithoutthe chamberriser

ChamberRiser

Sample Holder-Order Separately

Chambertop view

ChamberBase

Figure 1. H101-OLYMPUS-IX3-SVR - Components and Dimensions.

User Manual

March 2015

2. Sample Holders and Lid Adapters

2.1 Available Sample Holders

The following sample holders are available. H101-OLYMPUS-IX3-SVR features a lid adapter for each sample holder (in-

cluded in the sample holder code). Lid adapter fit into the chamber lid and is held in place by magnets embedded

within both chamber lid and lid adapter as illustrated in Figure 3, Point A. Figure 2 shows the available sample holders

and the corresponding lid adapters.

NOTE: Please contact **************** if you cannot find the sample holder you are looking for. We are constantly

adding new inserts to the list.

H101-1x35-M #1 35mm Petri-dish

H101-1xGS-M #1 1x3in. chamber slide

H101-1xLABTEK-M #1 Lab-Tek 1in.x2in. chambered cover glass

H101-1xLABTEK-II-M #1 Lab-Tek II 1in.x2in. chambered cover glass

H101-1x60-M #1 60mm Petri-dish

H101-1xT25-M #1 Nunc and Greiner T25 flask

H101-2x35-M #2 35mm Petri-dish

H101-2xGS-M #2 1x3in. chamber slides

H101-2xLABTEK-M #2 Lab-Tek 1in.x2in. chambered cover glass

H101-2xLABTEK-II-M #2 Lab-Tek II 1in.x2in. chambered cover glass

H101-2x60-M #2 60mm Petri-dish

H101-4x35-M #4 35mm Petri-dish

H101-GS35-M #1 1x3in. chamber slide and #2 35mm Petri-dish

H101-LABTEK-35-M #1 Lab-Tek 1in.x2in. chambered cover glass and #2 35mm Petri-dish

H101-LABTEK-II-35-M #1 Lab-Tek II 1in.x2in. chambered cover glass and #2 35mm Petri-dish

H101-LABTEK-60-M #1 Lab-Tek 1in.x2in. chambered cover glass and #1 60mm Petri-dish

H101-LABTEK-II-60-M #1 Lab-Tek II 1in.x2in. chambered cover glass and #1 60mm Petri-dish

H101-GS60-M #1 1x3in. chamber slide and #1 60mm Petri-dish

H101-6035-M #1 60mm Petri-dish and #1 35mm Petri-dish

H101-6MW 6-well plates, one MW LOCK required

H101-12MW 12-well plates, one MW LOCK required

H101-24MW 24-well plates, one MW LOCK required

H101-24MW-NUNC 24-well NUNC/GREINER plates, one MW LOCK required

H101-48MW 48-well plates, one MW LOCK required

H101-96MW

96-well plates, one MW LOCK required. It includes the insert for oil immer-

sion imaging.

MW-LOCK-22.5 Magnetic lock to hold the standard MW plates in the chamber

MW-LOCK-16.5 Magnetic lock to hold the low-profile MW plates in the chamber

User Manual

March 2015

4x35-M

GS35-M2x60-MGS60-MLABTEK-60-M

For #435 mm petridishes

For #1 1”x3”chamber slide andFor #260mm petridishes

For #1 60mm petridishand

For #1 Lab-Tek1”x2”chambered

#2 35mm petridishes#1 1”x3”chamber slidecover glass and #1 60mm petri dish

1x35-M6035-M

1xGS-M2xGS-M

LABTEK-II-60-M

For #1 35 mm petridish

For #235 mm petridishesandFor #1 1”x3”chamberslideFor #2 1”x3”chamberslide

For #1 Lab-Tek-II1”x2”chambered

#1 60mm petridishcover glass and #1 60mm petri dish

1x60-M

1xLABTEK-M1xLABTEK—II-M

2x35-M2xLABTEK-M

For #1 35 mm petridishFor #1 Lab-Tek1”x2”chamberedFor #1 Lab-TekII 1”x2”chamberedFor #235 mm petridishesFor #2 Lab-Tek1”x2”chambered

cover glass cover glass

cover glass

2xLABTEK-II-M

LABTEK-II-35-MLABTEK-35-M

H101-1xT25-MH101-6MW

For #2 Lab-Tek

For #1 Lab-TekII 1”x2”chamberedFor #1 Lab-Tek1”x2”chambered

For #1 Nuncand GreinerFor #1 6-well platesholder

1”x2”chamberedcover glass cover glass and #2 35mm petri dishescover glass and #2 35mm petri dishes

T25 flaskholder

H101-12MWH101-24MWH101-96MW

H101-24-NUNC-MWH101-48MW

For #1 12-well platesholderFor #1 24-well platesholderFor #1 96-well platesholder

For #1 24NUNC/GREINER-wellFor #1 48-well platesholder

platesholder

Figure 2. Available sample holders with corresponding lid adapters.

User Manual

March 2015

Lid Adapter

Lid

Figure 3. Assembly Lid Adapter to the Lid Chamber

3. Insertion of the Sample Feedback Temperature Sensor

Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H101-OLYMPUS-IX3-SVR

(see Figure 4, Frontal and 3D views).

Temperature Sensor dedicated opening

1. Frontalview2. 3D view

Figure 4. Insertion of the temperature sensor inside the chamber

4. Insertion of Sample Holder into Chamber

Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold-

er. To introduce a sample holder with the proper orientation, match the red dot on the holder to the one on the

chamber base, as illustrated in Figure 5.

H101-OLYMPUS-IX3-SVR

User Manual

March 2015

Insertthe adapterby

superimposingthe redpoints

Figure 5. Introduction of the Specimen Holder inside the Chamber Base.

User Manual

March 2015

5. Working with 35 or 60 mm Petri Dish – Spacing Rings and

Magnetic Locks

Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder.

Figure 6 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting

holder’s height.

MagneticLocksfor 35 and 60 mm petridishes.

Includedin the correspondingsample holder

Figure ic locks for 35 and 60 mm dish

NOTE: Magnetic locks are included with sample holder.

Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included. Figure 7

shows how to position the ring. Ring selection guide diagrams: rings for 35 and 60 mm dish -Figure 8 and Figure 9, re-

spectively.

Figure 7. 1) Insertion of the ring, 2) insertion of the 35 mm dish, 3) insertion of the magnetic lock.

NOTE: Spacing rings are included with sample holder.

User Manual

March 2015

Grainer-Petri35x10Ibidiμ-Dish35 mm-low

BD Falcon-35x10Willco-35x10Corning-35x10 mmMatTek-P35G-X14-X

Figure 8. Ring selection scheme for 35 mm dish.

Ibidiμ-Dish50 mm-low

MatTek-P50G-X30-X

BD Falcon-60x10Grainer-Petri 60x15

Corning-60x15 mm

Willco60x15

Figure 9. Ring selection scheme for 60 mm dish.

User Manual

March 2015

6. Working with 1x3’’ and 1x2’’ chamber slides - magnetic

locks

Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder.

NOTE: Magnetic locks are included with sample holder.

Figure 10. Magnetic lock for 1'' x 3'' chamber slide.

Figure 11. Magnetic lock for 1'' x 2'' chamber slide. To lock the slide, push simultaneously the buttons indicated with (3).

User Manual

March 2015

7. Working with MW Plates - Magnetic locks and Chamber ris-

Magnetic locks hold MW plates in place into sample holder illustrated in Figure 12

NOTE: Magnetic locks must be ordered separately (NOT INCLUDED). Product codes: MW-LOCK 22.5 and MW-LOCK 16.5.

Magneticlock

MW-22.5-LOCK. To be orderedseparately.

Typicallyusedfor 6, 12, 24, 48 MW

MW-16.5-LOCK. To be orderedseparately.

Typicallyusedfor 96 MW

Figure 12. Magnetic locks for MW plates. To be ordered separately.

Chamber Riser increases chamber’s height and is REQUIRED with 6, 12, 24, 48 MW plates, regardless of the Lid em-

ployed.

Figure 13 shows how to mount and screw the chamber riser. Screws location is indicated by letter A.

ChamberRiser

ChamberBase

Figure 13. Riser Assembly.

User Manual

March 2015

8. Connectors description

1. Gas Input. A single silicon tubing carries output gas from the Okolab Bubbler Column to the H101-OLYMPUS-

IX3-SVR. Silicon tubing connects to a gas input - brass opening - located on a corner of the H101-OLYMPUS-

IX3-SVR. See Figure 14 Point 1. Connect by gently pushing silicon tubing onto brass opening.

2. Water Input and Output. Two silicon tubing carry output and input water from the thermostatic bath to the

H101-OLYMPUS-IX3-SVR. See Figure 14 Points 2. Connect by gently pushing silicon tubing onto steel opening.

Match colors on chamber steel openings with ones on the thermostatic bath.

3. Water Input and Output. A single silicon tubing carries output water from the lid water jacket to the base

one. See Figure 14 Points 3. The silicon tubing connects the base and lid as shown in Figure 15.

Figure 14. Connectors.

Figure 15. Communication tubing between the base and chamber lid

User Manual

March 2015

9. Working with Perfusion

The Chamber Riser included with H101-OLYMPUS-IX3-SVR features 12 perfusion holes for the insertion of perfusion

tubing up to 2.5 mm in outer diameter. Small screws plug the perfusion holes when not in use. (Grub screws M3x6).

Remove small screws as necessary before introducing perfusion tubing.

Figure 16 shows location of perfusion holes.

Perfusiondedicatedopening

l view2.3D view

Figure 16. Perfusion

10. Connection of the Chamber with adapter

Follow the steps shown in the images of Figure 17 and listed below in order to correctly install the chamber onto XY

stage:

1. See Figure 17 Images 1 and 2 for placing the captive screws in the proper housings

2. Place the chamber on the adapter as shown in Figure 17 Image 3

3. Use a 1.5mm metric Allen Wrench to tighten the four captive screws in the proper housings (A in Figure 17 Image

4. Figure 17 Image 5 shows the chamber fixed on the adapter.

User Manual

March 2015

Captive Screw

Figure 17. How to assemble the chamber to the adapter.

11. Connecting the Chamber with XY stage

Once you have assembled the chamber with adapter you can place it on the XY stage, following the steps shown in

Figure 18.

5. Place the adapter on the stage (See Figure 18 Image 1).

6. Follow the instructions shown in Image 2 of Figure 18 for placing socket head cap screws; use the provided Allen

wrench to tighten the socket head cap screws and to fasten the adapter on the stage. Screws housings are indi-

cated with letter A in Image 2 of Figure 18.

User Manual

March 2015

ScrewM3x6

Figure 18. Connecting the Chamber with XY Stage